The Alsteens group focuses on viral and immune biophysics, using cutting-edge biophysical techniques to explore the molecular interactions that govern fundamental processes in biology. Our research is centered on understanding the nanomechanical properties of molecular interactions at the single-molecule level, specifically how these interactions occur in physiologically relevant environments. By recreating conditions that closely mimic the complexity of living systems, we aim to gain unprecedented insights into the dynamic processes occurring at the surface of mammalian cells.
A major emphasis of our work lies in deciphering the molecular mechanisms underlying critical events such as viral binding, entry, and internalization. By studying how viruses attach to and infect host cells, we uncover the biophysical principles that govern their infectivity and tropism. In parallel, we investigate immune cell interactions, focusing on how receptor-ligand engagements shape immune responses and the molecular pathways they activate.
Our approach combines innovative single-cell biophysical methodologies, such as atomic force microscopy (AFM) and optical tweezers (OT), with complementary optical microscopy techniques, including confocal microscopy, raster image correlation spectroscopy (RICS), and super-resolution microscopy (STED). This integrative strategy allows us to unravel the interplay between cellular architecture, molecular recognition, and functional outcomes. By bridging molecular-level insights with cellular processes, we aim to contribute to a deeper understanding of host-pathogen dynamics and the intricate mechanisms sustaining immune function, paving the way for novel therapeutic interventions.
Our research is conducted in collaboration with numerous national and international research groups, fostering a multidisciplinary and global approach to addressing complex scientific questions. The laboratory’s activities are supported by a combination of national funding (Welbio, EoS, FNRS, and UCLouvain) and international grants, including prestigious support from the European Research Council (ERC).
